Single-Cell Transcript Analysis of Pancreas Development
نویسندگان
چکیده
منابع مشابه
transcript analysis of heat shock protein 72 in vitrified 2-cell mouse embryos and subsequent in vitro development
objective: the aim of the study was to compare the effects of two different concentrations of cryoprotectants by cryotopvitrification on survival, developmental capacity and heat shock protein 72 (hsp72) expression of two-cell mouse embryos. materials and methods: in this experimental study, transcript analysis of hsp72 gene was performed on non-vitrified and vitrified 2-cell mouse embryos via ...
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Gene expression levels vary greatly within similar cells, even within clonal cell populations [1]. These spontaneous expression differences underlie cell fate diversity in both differentiation and disease [2]. The mechanisms responsible for generating expression variability are poorly understood. Using single-cell transcriptomics, we show that transcript variability emerging during Dictyosteliu...
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To understand organ function, it is important to have an inventory of its cell types and of their corresponding marker genes. This is a particularly challenging task for human tissues like the pancreas, because reliable markers are limited. Hence, transcriptome-wide studies are typically done on pooled islets of Langerhans, obscuring contributions from rare cell types and of potential subpopula...
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Human pancreatic islets consist of multiple endocrine cell types. To facilitate the detection of rare cellular states and uncover population heterogeneity, we performed single-cell RNA sequencing (RNA-seq) on islets from multiple deceased organ donors, including children, healthy adults, and individuals with type 1 or type 2 diabetes. We developed a robust computational biology framework for ce...
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BACKGROUND Diabetes mellitus is characterized by loss or dysfunction of insulin-producing β-cells in the pancreas, resulting in failure of blood glucose regulation and devastating secondary complications. Thus, β-cells are currently the prime target for cell-replacement and regenerative therapy. Triggering endogenous repair is a promising strategy to restore β-cell mass and normoglycemia in dia...
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ژورنال
عنوان ژورنال: Developmental Cell
سال: 2003
ISSN: 1534-5807
DOI: 10.1016/s1534-5807(03)00035-2